Evaluation of an Image Analysis Device for Screening Urine Cultures

28 January 2017

While advancements have been made in some areas of pathology with diagnostic materials being screened using image analysis technologies, the reporting of cultures from agar plates remains a manual process. We compared the results for 2,163 urine cul­tures read by a reference panel of microbiologists, by the routine laboratory process, and by an automated plate reading system, APAS (LBT Innovations Ltd., South Australia).

APAS detected colonies with a sensitivity of 99.1 % and a specificity of 99.3% on blood agar, while on MacConkey agar, the colony detection sensitivity was 99.4% with a specificity of 99.3%. The device’s ability to enumerate growth had an accuracy of 89.2%, and the morphological identification of colonies showed a high level of perfor­mance for the colony types typical of Escherichia coli and other enteric bacilli. On blood agar, lactose-fermenting colonies were morphologically identified with a sensitivity of 98.9%, while on MacConkey agar they were identified with a sensitivity of 99.2%.

In this first clinical evaluation, APAS demonstrated high performance in the detection, enumeration, and colony classification of isolates compared with that for conventional plate-reading methods.

The device found all cases reported by the laboratory and detected the most commonly encountered organisms found in urinary tract infections.

Read more about ‘Using APAS Independence for Automated Imaging and Assessment of Urine Culture Plates’ here.

To read the full article click here.

Journal of Clinical Microbiology, 2016